THE IN VITRO DIFFERENTIATION OF MONONUCLEAR PHAGOCYTES
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چکیده
منابع مشابه
The in Vitro Differentiation of Mononuclear Phagocytes
Mouse mononuclear phagocytes cultivated in 50 per cent newborn calf serum medium pinocytize actively and form large numbers of phase-dense granules as well as three hydrolytic enzymes. When such cells are then placed in 1 per cent newborn calf serum they illustrate (a) a low level of pinocytic activity, (b) a shrinkage in granule size, and (c) a loss in cell protein, acid phosphatase, beta-gluc...
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The in vitro differentiation of homogeneous populations of monocyte-like cells from the unstimulated mouse peritoneal cavity is described. Under the conditions employed, a progressive increase in cell size occurs without significant cell division. This process is characterized morphologically by the accumulation of phase-dense and neutral red-positive granules, mitochondria, and lipid droplets....
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In Vitro Synthesis and Secretion of Lysozyme by Mononuclear Phagocytes
Pure cultures of three types of mononuclear phagocytes-mouse peritoneal macrophages, unstimulated or after thioglycollate stimulation, and human monocytes-synthesize and secrete large amounts of lysozyme in vitro. The macrophage lysozyme is indistinguishable from authentic lysozyme in its ability to lyse M. lysodeikticus, inhibition by specific antisera, a similar size of 14,000 and cationic ch...
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Little is known about how mononuclear phagocytes (MP) are cleared from sites o f inflammation as inflammatory lesions resolve. In this study, the possibility that MP could be cleared from tissues by migrating across endothelium in the basal to apical direction was investigated. In an in vitro model o f a blood vessel wall consisting of human umbilical vein endothelial cells (HUVEC) grown on amn...
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ژورنال
عنوان ژورنال: Journal of Experimental Medicine
سال: 1966
ISSN: 1540-9538,0022-1007
DOI: 10.1084/jem.123.4.747